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PINO LABORATORY

Neuropharmacology research at the University of Atacama

 

THE LAB

Lab Experiment

The research interests of our group include the study of new regulatory mechanisms of monoamine neurotransmission and how alterations in monoamine homeostasis contribute to human disease (e.g. Parkinson’s disease, depression, anxiety, attention deficit hyperactivity disorder, and drug addiction). We are particularly interested in learning how different G protein-coupled receptors signaling pathways modulate the function of brain monoamine transporters (DAT, SERT, NET) and how this mechanism influences the development and progression of mental disorders associated with an imbalance in monoamine levels.

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Additionally, in collaboration with Dr. Gonzalo Torres from CUNY we have initiated a new line of investigation related with the regulation of serotonin transporter (SERT) by G-protein coupled receptors through a mechanism Gβγ-dependent. Thus, we have evidenced that SERT is a new target of Gβγ signaling. Accordingly, we have observed that activation of Gβγ potentiate amphetamine-induced 5-HT efflux in hippocampal slices, and this efflux is inhibited in the presence of a Gβγ inhibitor. Thus, we hypothesized that the interaction between monoamine transporters (DAT, SERT and NET) and Gβγ promotes the release of monoamines through DAT, SERT and NET. Consequently, these studies will define not only the role that Gβγ plays in the addictive properties of amphetamine and its analogues, but also the contribution of Gβγ to monoamine homeostasis. The long-term goal of my project is to identify novel therapeutic targets that can be used in the treatment of neuropsychiatric disorders, including depression and drug addiction.

 

NEWS AND EVENTS

Lab News and Events

OFERTA DE EMPLEO

16 de noviembre de 2020

Se ofrece tesis de pregrado remunerada para estudiantes de Bioquímica, Química y Farmacia, Ciencias Biológicas y/o psicología dentro del marco del proyecto Fondecyt #11191049 relacionado con la Regulación del Transportador de Serotonina por GPCRs en un modelo animal de depresión. Durante el desarrollo del presente proyecto l@s estudiantes realizarán ensayos conductuales para medir anhedonia y ansiedad, junto con medidas de la función del transportador de serotonina en rebanadas de cerebro y emplearán técnicas de RT-PCR para medir marcadores inflamatorios e  inmunoblots para evaluar la expresión de proteínas relacionadas con la mantención de la homeostasis de serotonina.
Para cualquier información contactar al email jose.pino@uda.cl

Test Tubes

I CONGRESO DE ESTUDIANTES INVESTIGADORES DE LAS CIENCIAS DE LA SALUD

30 de noviembre de 2020

La Facultad de Ciencias de la Salud de la Universidad de Atacama esta organizazinado el I Congreso de Estudiantes Investigadores de las Ciencias de la Salud. Cabe señalar que, los link para la inscripción a los talleres estarán disponibles desde el 16 al 18 de noviembre en la página www.salud.uda.cl/congreso, además indicar que solo se podrán inscribir a un taller ya que serán con cupos limitados. 

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NEW PUBLICATION

1 de marzo de 2020

Dr. Pino has collaborated in the following  research published in Experimental Neurology.

Reference:

Dimitri N Koutzoumis, Macarena Vergara, Jose Pino, Julia Buddendorff, Habibeh Khoshbouei, Ronald J Mandel, Gonzalo Torres (2020). Alterations of the Gut Microbiota with Antibiotics Protects Dopamine Neuron Loss and Improve Motor Deficits in a Pharmacological Rodent Model of Parkinson's disease. Exp Neurol 325:113159. Available at: https://pubmed.ncbi.nlm.nih.gov/31843492/

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